pLVX-DD-tdTomato Reporter载体

LOCUS       pLVX-DD-tdTomato_Reporter   9200 bp    DNA   circular             6-NOV-2009COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Cat. No. 631753,631754FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=packaging\signal     misc_feature    5168..5759                     /label=WPRE     rep_origin      complement(7068..7741)                     /label=pUC\origin     CDS             complement(7886..8882)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     misc_feature    2028..2151                     /label=cPPT/CTS     LTR             5962..6598                     /label=3'\LTR     misc_feature    4026..4534                     /label=PGK\Promoter     misc_feature    4555..5154                     /label=Puro(R)     misc_feature    2195..2234                     /label=MCS     CDS             2247..2570                     /label=DD     CDS             2577..4004                     /label=tdTomatoBASE COUNT     2262 a      2444 c      2487 g      2007 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg      2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag      2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg      2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag      2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc tcagatgagt gtgggtcaga      2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca      2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg      2581 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct      2641 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca      2701 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc      2761 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg      2821 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg      2881 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca      2941 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca      3001 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga      3061 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct      3121 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca      3181 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc      3241 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct      3301 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg      3361 agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc cgcccctacg      3421 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg      3481 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca      3541 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact      3601 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga      3661 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga      3721 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg      3781 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga      3841 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc      3901 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg      3961 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg gccgcgactc      4021 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt      4081 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac      4141 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac      4201 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca      4261 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa      4321 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca      4381 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg      4441 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc      4501 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc      4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc      4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac      4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc      4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc      4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg      4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg      4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc      4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc      5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc      5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt      5161 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta      5221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc      5281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga      5341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac      5401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc      5461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc      5521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg      5581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc      5641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc      5701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg      5761 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca      5821 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca      5881 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac      5941 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc      6001 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca      6061 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag      6121 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc      6181 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta      6241 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga      6301 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact      6361 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc      6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct      6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga      6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag      6601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag      6661 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat      6721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac      6781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa      6841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat      6901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc      6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg      7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag      7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc      7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag      7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga      7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc      7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg      7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt      7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca      7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca      7561 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag      7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca      7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg      7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa      7801 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta      7861 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag      7921 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga      7981 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac      8041 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc      8101 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta      8161 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac      8221 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat      8281 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa      8341 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg      8401 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag      8461 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc      8521 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct      8581 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat      8641 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg      8701 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc      8761 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta      8821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg      8881 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat      8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc      9001 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat      9061 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta      9121 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata      9181 tgtactacaa acttagtagt //