“英拜为您实验加速”
技术服务网址：http://www.yingbio.com/
服务热线：400-696-6643、 18019265738
邮箱：daihp@yingbio.com 、 huizhang1228@foxmail.comSerum & Plasma 384HC miScript miRNA PCR Array
血清和血浆miRNA 384HC PCR芯片 | Product | Species | Technology | Cat. No. |
| Serum & Plasma 384HC miScript miRNA PCR Array | Human | miRNA Expression | MIHS-3106Z |
The Human Serum & Plasma 384HC miScript miRNA PCR Array profiles the expression of 372 miRNAs detectable in serum and plasma using the miScript PCR System. This high-content array also includes the 84 miRNAs detectable and differentially expressed in serum, plasma, and other bodily fluids that are found on the Human Serum & Plasma miScript miRNA PCR Array. The serum and plasma 384HC array provides disease and toxicology researchers with a convenient way to quickly analyze the extracellular, circulating miRNAs most relevant to pathophysiological conditions. The discovery of miRNAs in serum and plasma, and their apparently regulated levels there, suggest a mechanistic role for these master regulatory molecules in both normal and pathophysiological processes. Their presence in serum and plasma samples provides readily available source material for research purposes. The profiling results from this array can potentially serve as a useful molecular marker for heart and liver injury or disease, atherosclerosis, diabetes, and a number of organ-specific cancers. This array can also be used with miRNeasy Serum/Plasma Spike-In Control (catalog number 219610). When spiked into the sample before nucleic acid isolation and detected with the control assay, it provides a more useful normalization method for this application than the typical reference short noncoding RNAs, which are not normally detected in serum. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs found in serum and plasma can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
血清和血浆miRNA 384HC PCR芯片用于研究血清、血浆和其他体液中可见的和差异表达的372个 miRNA的表达。这个芯片为疾病和毒理学研究人员提供了一个快速方便的方法来分析与病理生理条件最相关的miRNA。这些miRNAL来自基于已发表的文献显示与血清表达水平和特定疾病相关得miRNA。这些主要调控分子在血清和生物体液中以及它们的表面监管水平的发现,表明miRNA在正常和病理生理过程中的机械作用。他们的非侵袭性,样本获得的容易使得它们为研究目的提供了现成的素材。这个芯片的分析结果可以作为心脏和肝脏损伤或疾病、动脉粥样硬化、糖尿病、瀑特异癌症有用的分子标记。其他常规存在于血清的miRNA作为成功检测miRNA的表达的阳性对照。这个芯片也可以使用miRNeasy Serum/Plasma Spike-In控制(产品目录号219610)。对照试验为这个程序提供了一个比典型参考更有用的归一化方法。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地分析高度研究的miRNA的表达。
miScript miRNA PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和ZL。
提供96孔板——384 -(4 x 96)板,和100孔板
资料下载：
QIAGEN实时定量PCR芯片技术服务资料.pdf
附件下载 (下载 1 次)
请 [登录] 后再下载!
