Human Apolipoprotein AII ELISA Kit（载脂蛋白Apo-AII酶联免疫试剂盒）

Introduction
Apolipoprotein A-II (Apo A-II) is the second most abundant apolipoproteins in human plasma HDL, comprising about 25% of the protein mass. After being synthesized by the liver and intestine as a preprotein containing 100 amino acids, Apo A-II is processed to 77 amino acids in the mature plasma protein (1 - 3). Apo A-II is found in plasma as a monomer, homodimer of 17.4 kDa, or heterodimer with Apo E and Apo D (4 - 7). It has been reported that Apo A-II plays roles in HDL remodeling, cholesterol efflux, modulating HDL interaction with enzymes and receptors, triglyceride metabolism, and atherosclerosis (7 - 12). Apo A-II is inversely associated with risk of future coronary artery disease (13). Serum levels of an isoform of Apo A-II have been identified as a potential marker for prostate cancer (14).
 
Principle of the Assay
The AssayMax Human Apo A-II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human apo A-II in plasma, serum, urine, milk, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human apo A-II in less than 4 hours. A monoclonal antibody specific for human apo A-II has been pre-coated onto a 96-well microplate with removable strips. Apo A-II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human apo A-II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.