Beta-Actin内参抗体, 抗Beta-Actin单抗, Beta-Actin小鼠源单克隆抗体

anti-beta-actin-mAb.pdf

Anti β-Actin Monoclonal Antibody       说明书下载 Calatog Number: LT0427 Lot Number: 411005100207 Isotype: Mouse IgG2b Clonality: BA3R Quantity: 50 μg Immunogen: β-Actin N-terminal peptide-KLH conjugates Specificity: Recognizes native and denatured forms of β-Actin (~42kDa) Cross Reactivity: β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IS, WB Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks Images: Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)