GAPDH内参抗体, 抗GAPDH单抗, GAPDH小鼠源单克隆抗

anti-gapdh-mAb.pdf

Anti GAPDH Monoclonal Antibody       说明书下载 Calatog Number: LT9995 Lot Number: 411005100211 Isotype: Mouse IgG1 Clonality: GA1R Quantity: 50 μg Immunogen: Recombinant GAPDH Specificity: Recognizes native and denatured forms of GAPDH (~37kDa) Cross Reactivity: GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IS, WB Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks Images: Fig. LEFT: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) td>