His标签抗体（抗His单抗）, Anti-His Tag Monoclonal Antibody

anti-his-mAb.pdf

Anti His Monoclonal Antibody       说明书下载 Calatog Number: LT0424 Lot Number: 411005100202 Isotype: Mouse IgG2b Clonality: HIS.H8 Quantity: 50 μg Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IP, IS, WB Sensitivity: WB (with ECL): 1-2ng of His-tagged fusion proteins Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks Images: Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue) Fig. 02 Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL) Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)