HRP标记His标签抗体 His-Tag Antibody HRP
- 型号：ICP0851
- 产地：中国大陆
- 供应商：南宁市蓝光生物技术有限公司
- 供应商报价：1750
- 标签：HRP标记His标签抗体 His-Tag Antibody HRP,生物试剂,生物制剂,供应HRP标记His标签抗体 His-Tag Antibody HRP,南宁市蓝光生物技术有限公司
产品库
| Product Description | This high affinity His-tag antibody HRP conjugate is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH ａnd affinity-purified with the HHHHHH peptide. The antibody is directly conjugated to peroxidase (HRP) ａnd is useful for detection ａnd localization of His-tag proteins. | |  Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate ａnd enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 µL of His-tag protein in lysate (original cell lysate) 3: 10 µL of His-tag protein in lysate (after IP) 4: 10 µL of the His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag (ICP0850) from 2 mL of the original lysate  A: 5 µg of His-tag fusion protein (50 kDa) B: 20 µg His-tag fusion protein (50 kDa) |
| Species | Rabbit | ||
| Formulation | 250 µg/mL in PBS, 50% glycerol | ||
| Immunogen | Polyhistidine peptide (HHHHHH) KLH conjugates | ||
| Purification | Antigen-specific affinity chromatography | ||
| Specificity | The antibody recognizes His-tag proteins. | ||
| Applications | ELISA (1:2500); WB (0.25-1.0 µg/mL); IHC (5 µg/mL) | ||
| Scientific Description | Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix. | ||
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
