裂谷热病毒荧光PCR检测试剂盒

裂谷热病毒荧光PCR检测试剂盒

广州健仑生物科技有限公司

One tube multiplex for detection of Rift Valley fever virus and internal control.

单管多重检测裂谷热病毒和内部控制。

裂谷热病毒荧光PCR检测试剂盒

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

1、计数器测定法：
即用血细胞计数器进行计数。取一定体积的样品细胞悬液置于血细胞计数器的计数室内，用显微镜观察计数。由于计数室的容积是一定的(O.1mm3)，因而根据计数器刻度内的细菌数，可计算样品中的含菌数。本法简便易行，可立即得出结果。
本法不仅适于细菌计数，也适用于酵母菌及霉菌孢子计数。
2、电子计数器计数法:
电子计数器的工作原理是测定小孔中液体的电阻变化，小孔仅能通过一个细胞，当一个细胞通过这个小孔时，电阻明显增加，形成一个脉冲，自动记录在电子记录装置上。
该法测定结果较准确，但它只识别颗粒大小，而不能区分是否为细菌。因此，要求菌悬液中不含任何碎片。
3、活细胞计数法
常用的有平板菌落计数法，是根据每个活的细菌能长出一个菌落的原理设计的。取一定容量的菌悬液，作一系列的倍比稀释，然后将定量的稀释液进行平板培流感据培养出的菌落数，可算出培养物中的活菌数。此法灵敏度高，是一种检测污染活菌数的方法，也是目前国际上许多国家所采用的方法。使用该法应注意：①一般选取菌落数在30～300之间的平板进行计数，过多或过少均不准确；②为了防止菌落蔓延，影响计数，可在培养基中加入O．001％2，3，5一氯化三苯基四氮唑(TTC)；③本法限用于形成菌落的微生物。
广泛应用于水、牛奶、食物、药品等各种材料的细菌检验，是Z常用的活菌计数法。
4、比浊法
比浊法是根据菌悬液的透光量间接地测定细菌的数量。细菌悬浮液的浓度在一定范围内与透光度成反比，与光密度成正比，所以，可用光电比色计测定菌液，用光密度(OD值)表示样品菌液浓度。
此法简便快捷，但只能检测含有大量细菌的悬浮液，得出相对的细菌数目，对颜色太深的样品，不能用此法测定。
5、测定细胞重量法
此法分为湿重法和干重法。湿重法系单位体积培养物经离心后将湿菌体进行称重；干重法系单位体积培养物经离心后，以清水洗净放人干燥器加热烘干，使之失去水分然后称重。
此法适于菌体浓度较高的样品，是测定丝状真菌生长量的一种常用方法。
6、测定细胞总氮量或总碳量
氮、碳是细胞的主要成分，含量较稳定，测定氮、碳的含量可以推知细胞的质量。此法适于细胞浓度较高的样品。

1, the counter measurement method:
That is counted with a hemocytometer. A certain volume of sample cell suspension was placed in the counting chamber of a hemocytometer and the count was observed with a microscope. Since the volume of the counting chamber is constant (0.1 mm3), the number of bacteria in the sample can be calculated based on the number of bacteria in the counter scale. This Law is simple and straightforward and gives immediate results.
This Law is not only suitable for counting bacteria, but also for counting yeast and mold spores.
2, electronic counter counting method:
The working principle of the electronic counter is to measure the change of the resistance of the liquid in the small hole. The small hole can only pass through one cell. When a cell passes through the small hole, the resistance increases obviously to form a pulse and automatically record on the electronic recording device.
The method is more accurate determination of the results, but it only recognizes the size of the particles, but can not distinguish whether it is bacteria. Therefore, it is required that the bacterial suspension does not contain any debris.
3, live cell counting method
Commonly used plate colony counting method is based on the principle that each living bacteria can grow a colony. Take a certain volume of bacterial suspension for a series of multiple dilution, and then the quantitative dilution of plate culture according to the number of colonies cultured to calculate the viable count in the culture. This method is highly sensitive, is a method of detecting viable bacteria, but also the method used by many countries in the world. The use of the law should be noted: ① generally selected colony count between 30 to 300 plates count, too much or too little are not accurate; ② In order to prevent the spread of colonies affect the count can be added to the medium O. 001% 2,3,5 triphenyltetrazolium chloride (TTC); ③ This law is limited to the formation of colonies of microorganisms.
Widely used in water, milk, food, medicines and other materials of bacteria test, is the most commonly used viable count method. The company is located in:
4, turbid method
Turbidimetric method is based on the amount of bacterial suspension of indirect light to determine the number of bacteria. Bacterial suspension concentration within a certain range is inversely proportional to the light transmittance, and optical density is proportional to, therefore, the bacteria can be measured using a photoelectric colorimeter, with optical density (OD value) that the sample concentration.
This method is quick and easy, but can only detect the suspension containing a large number of bacteria, the relative number of bacteria obtained, the color of the sample is too deep, this method can not be determined.
5, determination of cell weight method
This method is divided into wet weight method and dry weight method. Method wet weight based unit volume of culture is weighed after centrifugation was wet cells; Department of dry weight per unit volume of the culture process by centrifugation, washed with water, heating and drying to release the dryer, so that loss of moisture and then weighed .
This method is suitable for higher bacterial concentration of samples, is to determine the amount of filamentous fungus growth of a common method.
6, determine the total amount of total cellular nitrogen or cells
Nitrogen, carbon is the main component of the cell, the content is more stable, the determination of nitrogen, carbon content can infer cell quality. This method is suitable for high cell concentration samples.