| | Anti GST Monoclonal Antibody | Calatog Number: | LT0423 | | Lot Number: | 411005100205 | | Isotype: | Mouse IgG2a | | Clonality: | GST.B6 | | Quantity: | 50 μg | | Immunogen: | GST protein | | Specificity: | Recognizes native and denatured forms of purified GST or GST fusion proteins | | Purification: | Protein A affinity chromatography from mouse ascites fluid | | Applications: | Dot, ELISA, IS, IP, WB | | Sensitivity: | WB （with ECL）: 10-50ng of purified GST or GST fusion proteins | | Working Condition: | WB （with ECL）: 1:1000-3000 dilution （incubate for 1 hour at room temperature）
IS: 1:500-2000 dilution
For best results with other assays （e.g.: Dot, ELISA, IP, etc）, please determine optimal working dilution by titration test | | Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks | | Images: | 
Fig. 1:5000 （0.2μg/mL） Ab dilution probed against BL-21 bacteria lysate expressing the GST protein | |
