Product Name:Human Choline dehydrogenase, mitochondrial, CHDH ELISA KIT
Packing:96T
Catalog No.:ELI-33845h
Gene Name:CHDH
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Choline dehydrogenase(mitochondrial(CHDH
Alternative Name:CHDH,Choline dehydrogenase(mitochondrial(CHDH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Choline dehydrogenase, mitochondrial, CHDH ELISA KIT allows for the in vitro quantitative determination of Choline dehydrogenase(mitochondrial(CHDH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Choline dehydrogenase, mitochondrial, CHDH ELISA KIT has been pre-coated with an Choline dehydrogenase(mitochondrial(CHDH antibody specific to Choline dehydrogenase(mitochondrial(CHDH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Choline dehydrogenase(mitochondrial(CHDH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Choline dehydrogenase(mitochondrial(CHDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Choline dehydrogenase(mitochondrial(CHDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
