Human Ceramide kinase, CERK ELISA KIT

Human Ceramide kinase, CERK ELISA KIT

Product Name:Human Ceramide kinase, CERK ELISA KIT
Packing:96T

Catalog No.:ELI-25147h

Gene Name:CERK

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Ceramide kinase(CERK

Alternative Name:CERK,Ceramide kinase(CERK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Ceramide kinase, CERK ELISA KIT allows for the in vitro quantitative determination of Ceramide kinase(CERK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Ceramide kinase, CERK ELISA KIT has been pre-coated with an Ceramide kinase(CERK antibody specific to Ceramide kinase(CERK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ceramide kinase(CERK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ceramide kinase(CERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ceramide kinase(CERK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!