Search name
lentiGuide-Puro,Plasmid lentiGuide-Puro,lentiGuide-Puro vectorBacterial Resistance:Ampicillin
Growth Strain:
Use:CRISPR-CAS25-sgRNA plasmid
lentiGuide-Puro plasmid information
Vector backbone Custom
Backbone size w/o insert (bp) 9482
Vector type Mammalian Expression, Lentiviral, CRISPR
Selectable markers Puromycin
Gene/Insert name S. pyogenes sgRNA cassette
Alt name S. pyogenes CRISPR customizable RNA element
Species Synthetic
Insert Size (bp) 100
Promoter hU6
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Growth instructions Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
Copy number High Copy
Cloning method Restriction Enzyme
5′ cloning site BsmBI (not destroyed)
3′ cloning site BsmBI (not destroyed)
5′ sequencing primer hU6-F
3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')
Gene/Insert name Puromycin resistance
Alt name puromycin N-acetyl-transferase
Alt name PAC
Insert Size (bp) 600
Promoter Ef1-a
Cloning method Restriction Enzyme
5′ cloning site BsiWI (not destroyed)
3′ cloning site MluI (not destroyed)
5′ sequencing primer EF-1a-F
3′ sequencing primer WPRE-R
武汉维克赛思科技有限公司
地址：武汉东湖新技术开发区东信路光谷创业街10栋
联系人：童经理
联系电话：13118103892
Email: info@vecscience.com
微信：13118103892
QQ: 3128136151
