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Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F32356

产品信息
  • Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F32356
    Catalog
    Size
    Price
    LS-F32356-1
    1 plate
    $690 USD
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    SpecificationsPublicationsReviewsImagesPopular CSTB / Cystatin B / Stefin B Elisa Kits
    Mouse CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F11270
    Type: Quantitative Sandwich ELISA
    Format: 96-Well Strip Plate
    Reactivity: Mouse
    Range: 0.156-10 ng/ml
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Serum, Plasma

    Rat CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F18665
    Type: Quantitative Sandwich ELISA
    Format: 96-Well Strip Plate
    Reactivity: Rat
    Range: 0.156-10 ng/ml
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Serum, Plasma

    Human/Rat CSTB / Cystatin B / Stefin B ELISA Kit (Cell-Based ELISA) - LS-F2334
    Type: Qualitative Cell-Based ELISA
    Format: 96-Well Microplate
    Reactivity: Human, Rat
    Range: Positive/Negative
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Adherent Cell Cultures

    Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F2885
    Type: Quantitative Sandwich ELISA
    Format: 96-Well Microplate
    Reactivity: Human
    Range: 62.5-4000 pg/ml
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Breast Milk, Cell Culture Supernatants, Saliva, Serum, Urine, Plasma

    Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F6573
    Type: Quantitative Sandwich ELISA
    Format: 96-Well Strip Plate
    Reactivity: Human
    Range: 0.313-20 ng/ml
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Serum, Tissue Homogenates, Plasma
    Product DescriptionLS-F32356 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CSTB / Cystatin B / Stefin B in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CSTB / Cystatin B / Stefin B as low as 0.094 nanograms per millilter.
    About CSTB / Cystatin B / Stefin B
    The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. P04080  NM_000100 NP_000091.1

     CSTB ElisaKit, CPI-B ElisaKit, EPM1 ElisaKit, EPM1A ElisaKit, PME ElisaKit, Stefin B ElisaKit, Stefin-B ElisaKit, ULD ElisaKit, Cystatin b ElisaKit, Cystatin B (stefin B) ElisaKit, Cystatin-B ElisaKit, STFB ElisaKit

    More CSTB / Cystatin B / Stefin B Products
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    SpecificationsType
     
    Sandwich ELISA (enzyme-linked immunosorbent assay) kit
    Target
     
    CSTB / Cystatin B / Stefin B
    Reactivity
     
    Human
    Manual
     

    Intended Sample Types
     
    Plasma, Serum, Tissue Homogenates
    Format
     
    96-Well Strip Plate
    Detection
     
    Colorimetric - 450nm (TMB)
    Measurement
     
    Quantitative
    Detection Range
     
    0.156 - 10 ng/ml
    Sensitivity
     
    0.094 ng/ml
    Precision
     
    Intra-Assay: CV<8% Inter-Assay: CV<10%
    Storage
     
    Store at 4°C. Stable for 6 months.
    Quality Assurance
     
    Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
    Kit Components
     
    • Coated 96-well Strip Plate
    • Detection Antibody Diluent
    • Biotinylated Detection Antibody (100x)
    • HRP-Streptavidin Conjugate (100x)
    • HRP-Streptavidin Conjugate Diluent
    • Sample Diluent
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (25x)
    • Standard (Lyophilized)
    • Adhesive Plate Sealers

    Background
     
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CSTB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CSTB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CSTB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CSTB can be calculated.
    Restrictions
     
    For research use only.
    Guarantee
     
    This elisa kit carries the LSBio Guarantee.
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