Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F24477

Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F24477
Catalog
Size
Price
LS-F24477-1
1 plate
$690 USD
Request Bulk
Add to Wish List
Add to CartPerformance Guaranteed



SpecificationsPublicationsReviewsImagesPopular CSTB / Cystatin B / Stefin B Elisa Kits
Mouse CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F11270
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Rat CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F18665
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human/Rat CSTB / Cystatin B / Stefin B ELISA Kit (Cell-Based ELISA) - LS-F2334
Type: Qualitative Cell-Based ELISA
Format: 96-Well Microplate
Reactivity: Human, Rat
Range: Positive/Negative
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F2885
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 62.5-4000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Breast Milk, Cell Culture Supernatants, Saliva, Serum, Urine, Plasma

Human CSTB / Cystatin B / Stefin B ELISA Kit (Sandwich ELISA) - LS-F6573
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

---

Product DescriptionLS-F24477 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CSTB / Cystatin B / Stefin B in samples of Cell Culture Supernatants, Plasma, Serum and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of CSTB / Cystatin B / Stefin B as low as 10 picograms per milliliter.
About CSTB / Cystatin B / Stefin B
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. Uniprot: P04080 NCBI: NM_000100 NP_000091.1

Synonyms: CSTB ElisaKit, CPI-B ElisaKit, EPM1 ElisaKit, EPM1A ElisaKit, PME ElisaKit, Stefin B ElisaKit, Stefin-B ElisaKit, ULD ElisaKit, Cystatin b ElisaKit, Cystatin B (stefin B) ElisaKit, Cystatin-B ElisaKit, STFB ElisaKit

More CSTB / Cystatin B / Stefin B Products
Top of Page

---

SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
CSTB / Cystatin B / Stefin B
Reactivity
 
Human
Specificity
 
For the detection and quantification of endogenous levels of natural and/or recombinant Human Cystatin B proteins.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum, Urine
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
62.5 - 4000 pg/ml
Sensitivity
 
10 pg/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Standard (Lyophilized)
• Biotinylated Detection Antibody (100x)
• Avidin-Biotin-HRP Complex (100x)
• Sample Diluent
• Biotinylated Detection Antibody Diluent
• Avidin-Biotin-HRP Complex Diluent
• Wash Buffer (20x)
• TMB Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
Top of Page

---

Publications (0)Submit a Publication
Top of Page

---

Customer Reviews (0)Submit a Review
Top of Page

---

Images

 

 

Sandwich ELISA Platform Overview