现货供应，美国进口， MK-2206 dihydrochloride ，目录号S1078 ，CAS号:1032350-13-2， PI3K / mTOR / AKT ，AKT YZ剂，Selleck_详细资料

分子量(MW):	480.39
化学式:	C₂₅H₂₁N₅O.2HCl
溶解度:	DMSO ≥8mg/mL Water ≥20mg/mL Ethanol <1mg/mL
纯度:	99%
稳定性:	at -20℃ 2 years
CAS号:	1032350-13-2

分子量(MW):

480.39

化学式:

C₂₅H₂₁N₅O.2HCl

溶解度:

DMSO ≥8mg/mL Water ≥20mg/mL Ethanol <1mg/mL

纯度:

99%

稳定性:

at -20℃ 2 years

CAS号:

1032350-13-2

	C.U87MG cells were treated with vehicle or 1 μM MK-2206 for 1 hr, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control. D. Cells were treated with vehicle (control) or 1 μM MK-2206 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.
	After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of MK-2206 for 3h,followed by 15-minute stimolation of 100ng/ml EGF.
	Effect of selected agents on NOx and cGMP formation induced by AEA. Washed platelets (1.0109 platelets/mL), prewarmed at 378C with saline or 1mM SR1, 1mM SR2, 20 mMURB597 (URB), 1mMMK2206 (MK) or 20 mMLY294002 (LY), were incubated for 1min with 100 mML-arginine in the presence of 1.0 mMAEA. NOx (panel A) and cGMP (panel B) content were determined as detailed in Methods. Each bar represents the meanSD of five independent experiments carried out in triplicate. Student’s t-test: P <0.0001 versus none;P <0.0005; §P <0.005 versus AEA
	Confocal microscopy images of NO formation. DAF 2 DA-loaded washed platelets (1.0109 platelets/mL) were preincubated at 378C with saline (A), and then stimulated for 1min with 0.1 (B), 1.0 (C) or 10 (D) mM AEA. In Panel (E–F–G) washed platelets were preincubated with 1 mM SR1 (E), 1 Mm MK2206 (F) or 20 mM LY294002 (G), and then stimulated for 1min with 1.0 mM AEA. In panel (H) is reported the effect of 5 mg/mL collagen, used as a positive control. All the experiments were carried out in the presence of 100 mM L-arginine. NO formation was visualized by confocal microscropy as detailed in Methods.
	The AEA effect on eNOS phosphorylation. Washed platelets (1.0109 platelets/mL), preincubated at 378C with saline, 1 mM SR1, 1mM SR2, 20 mM LY294002, 1mM MK2206, 1.0mM EGTA, or 30 mM BAPTA/AM, were stimulated for 1 min with 1.0 mM AEA. At the end of incubation suitable aliquots were immunoblotted with anti-p-eNOSser1177 as detailed in Methods.Blots are representative of five independent experiments.
	Mean IL-8 concentrations determined by ELISA of the supernatants of HeLa cells infected with wild-type Salmonella. Kinase inhibitors are indicated on the x axis, and the target families of the inhibitors are indicated above each column. CEC, chelerythrine; Pim Inh, Pim-1 inhibitor 2. Inhibitors that significantly affected IL-8 production relative to the control (P < 0.05, Bonferroni post hoc test from one-way ANOVA) are indicated with an asterisk. Relative cell viability is also shown, as determined by reduction of XTT by viable cells. A450, absorbance at 450 nm.

C.U87MG cells were treated with vehicle or 1 μM MK-2206 for 1 hr, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control.

D. Cells were treated with vehicle (control) or 1 μM MK-2206 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.

After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of MK-2206 for 3h,followed by 15-minute stimolation of 100ng/ml EGF.

Effect of selected agents on NOx and cGMP formation induced by AEA. Washed platelets (1.0109 platelets/mL), prewarmed at 378C with saline or 1mM SR1, 1mM SR2, 20 mMURB597 (URB), 1mMMK2206 (MK) or 20 mMLY294002 (LY), were incubated for 1min with 100 mML-arginine in the presence of 1.0 mMAEA. NOx (panel A) and cGMP (panel B) content were determined as detailed in Methods. Each bar represents the meanSD of five independent experiments carried out in triplicate. Student’s t-test: P <0.0001 versus none;P <0.0005; §P <0.005 versus AEA

Confocal microscopy images of NO formation. DAF 2 DA-loaded washed platelets (1.0109 platelets/mL) were preincubated at 378C with saline (A), and then stimulated for 1min with 0.1 (B), 1.0 (C) or 10 (D) mM AEA. In Panel (E–F–G) washed platelets were preincubated with 1 mM SR1 (E), 1 Mm MK2206 (F) or 20 mM LY294002 (G), and then stimulated for 1min with 1.0 mM AEA. In panel (H) is reported the effect of 5 mg/mL collagen, used as a positive control. All the experiments were carried out in the presence of 100 mM L-arginine. NO formation was visualized by confocal microscropy as detailed in Methods.

The AEA effect on eNOS phosphorylation. Washed platelets (1.0109 platelets/mL), preincubated at 378C with saline, 1 mM SR1, 1mM SR2, 20 mM LY294002, 1mM MK2206, 1.0mM EGTA, or 30 mM BAPTA/AM, were stimulated for 1 min with 1.0 mM AEA. At the end of incubation suitable aliquots were immunoblotted with anti-p-eNOSser1177 as detailed in Methods.Blots are representative of five independent experiments.

Mean IL-8 concentrations determined by ELISA of the supernatants of HeLa cells infected with wild-type Salmonella. Kinase inhibitors are indicated on the x axis, and the target families of the inhibitors are indicated above each column. CEC, chelerythrine; Pim Inh, Pim-1 inhibitor 2. Inhibitors that significantly affected IL-8 production relative to the control (P < 0.05, Bonferroni post hoc test from one-way ANOVA) are indicated with an asterisk. Relative cell viability is also shown, as determined by reduction of XTT by viable cells. A450, absorbance at 450 nm.

您可能感兴趣的产品

GM0050SP1MNN 现货供应进口GM0050SP1MNN美国米顿罗

型号：GM0050SP1MNN
上海蓝季长期现货供应吖啶橙、美国分装

型号：10127-02-3
FLIR E4/E5/E6/E8 现货供应高性能美国菲力尔FLIR E4/E5/E6/E8红外热像仪

型号：FLIR E4/E5/E6/E8
40CDHMIRN24MC350C110 现货供应派克PARKER美国40CDHMIRN24MC350C1100...

型号：40CDHMIRN24MC350C110
PGM-3000气体检测仪--现货供应总代理美国RAE
PGM-3000气体检测仪--宏昌信现货供应总代理美国RAE

品牌：美国华瑞
现货供应 Hyclone 30071.03 美国血源
SQB-2.5t 现货供应SQB-2.5t美国AMCELLS传感器称重传感器厂家

型号：SQB-2.5t
SQB-1.5t 现货供应SQB-1.5t美国AMCELLS传感器称重传感器厂家

型号：SQB-1.5t
DEC-1.5t 现货供应DEC-1.5t美国MKCELLS传感器

品牌：美国zemic

型号：DEC-1.5t
现货供应优质级美国Sigma-D5879,二甲基亚砜

品牌：Sigma-

型号：D5879
现货供应优质级美国 D-山梨醇

型号：S6021