GFP标签抗体, 抗GFP绿色荧光单抗, GFP小鼠源单克隆抗体

anti-gfp-mAb.pdf

Anti GFP Monoclonal Antibody       说明书下载 Calatog Number: LT9994 Lot Number: 411005100210 Isotype: Mouse IgG1 Clonality: GF28R Quantity: 50 μg Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates Specificity: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IP, IS, WB Sensitivity: WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks Images: Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used