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Anti GFP Monoclonal Antibody
| Calatog Number: |
LT9994 |
| Lot Number: |
411005100210 |
| Isotype: |
Mouse IgG1 |
| Clonality: |
GF28R |
| Quantity: |
50 μg |
| Immunogen: |
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
| Specificity: |
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP |
| Purification: |
Protein A affinity chromatography from mouse ascites fluid |
| Applications: |
Dot, ELISA, IP, IS, WB |
| Sensitivity: |
WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins |
| Working Condition: |
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Storage: |
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| Images: |
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used |
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![Anti-GFP Tag Monoclonal Antibody [GF28R]<em>GFP标签抗体, 抗GFP绿色荧光单抗</em>,GFP小鼠源单克隆抗体](https://item.yiqi.com/pic/CovPic/4/default2x.png)
