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Anti Anti-DYKDDDDK（FLAG） Monoclonal Antibody
| Calatog Number: |
LT0420 |
| Lot Number: |
411005100203 |
| Isotype: |
Mouse IgG2b |
| Clonality: |
FG4R |
| Quantity: |
50 μg |
| Immunogen: |
DYKDDDDK (FLAG) synthetic peptide conjugated to KLH |
| Specificity: |
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins |
| Purification: |
Protein A affinity chromatography from mouse ascites fluid |
| Applications: |
Dot, ELISA, IS, IP, WB |
| Sensitivity: |
WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins |
| Working Condition: |
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Storage: |
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| Images: |
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2) |
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