Flag标签抗体, 抗Flag单抗, Flag小鼠源单克隆抗体

anti-flag-mAb.pdf

Anti Anti-DYKDDDDK（FLAG） Monoclonal Antibody       说明书下载 Calatog Number: LT0420 Lot Number: 411005100203 Isotype: Mouse IgG2b Clonality: FG4R Quantity: 50 μg Immunogen: DYKDDDDK (FLAG) synthetic peptide conjugated to KLH Specificity: Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IS, IP, WB Sensitivity: WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins Working Condition: WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks Images: Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)