Alternative Name
pCambia 1391Z, pCambia 1391Z Vector, pCambia 1391Z Plasmid, pCambia 1391Z map
pCambia1391Z Information
Plasmid type: plant expression vector
High copy / low copy: low copy
Promoter: CAMV 35S
Cloning methods: polyclonal sites, restrictive endonucleases
Carrier size: 11227 BP
5'sequencing primers and sequences: M13-F: TGTAAAACGACGGCCAGT
3'sequencing primers and sequences: M13-R: CAGGAAACAGCTATGAC
Carrier label: GusA
Carrier resistance: kanana and Hygromycin
Screening markers: HPTII
Note: -
Stability: stable expression
Composition type: non group molding
Virus / non virus: non virus
pCambia1391Z Sequence
LOCUS pCAMBIA1391Z 11227 bp ds-DNA circular SYN
DEFINITION Agrobacterium binary vector for plant transformation, with
hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the
pUC9 MCS.
ACCESSION AF234312
VERSION .
KEYWORDS pCAMBIA1391Z
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11227)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported from SnapGene Viewer
COMMENT The GenBank record was corrected by inserting a G at position 4743.
FEATURES Location/Qualifiers
source 1..11227
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS join(2..16,207..2024)
/codon_start=1
/product="beta-glucuronidase"
/label="GUS"
/label="This version of the gusA gene has a 5' extension
with a catalase intron to ensure expression in plants but
not bacteria."
/protein_id="
"
intron 17..206
/label="cat1 intron"
/label="castor bean catalase intron, modified"
CDS 2031..2048
/codon_start=1
/product="6xHis affinity tag"
/label="6xHis"
/translation="HHHHHH"
terminator 2083..2335
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
misc_feature 2357..2381
/label="RB T-DNA repeat"
/label="right border repeat from nopaline C58 T-DNA"
CDS 3681..4310
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label="pVS1 StaA"
CDS 4739..5812
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label="pVS1 RepA"
/label="
"
/protein_id="
"
misc_feature 6416..6556
/label="bom"
/label="basis of mobility region from pBR322"
rep_origin complement(6742..7330)
/direction=LEFT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7417..8211)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label="KanR"
/label="confers resistance to kanamycin"
/protein_id="
"
misc_feature 8636..8660
/label="LB T-DNA repeat"
/label="left border repeat from nopaline C58 T-DNA"
polyA_signal 8738..8912
/label="CaMV poly(A) signal"
/label="cauliflower mosaic virus polyadenylation signal"
CDS complement(8952..9977)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label="HygR"
/label="confers resistance to hygromycin"
promoter complement(10045..10722)
/label="CaMV 35S promoter (enhanced)"
/label="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 10936..10966
/label="lac promoter"
/label="promoter for the E. coli lac operon"
protein_bind 10974..10990
/bound_moiety="lac repressor encoded by lacI"
/label="lac operator"
/label="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10998..11014
/label="M13 rev"
/label="common sequencing primer, one of multiple similar
variants"
CDS 11010..11207
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of?beta-galactosidase"
/label="lacZ-alpha"
/protein_id="
"
/translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
ASWRNSEEARTDRPSQQLRSL"
misc_feature 11027..11062
/label="MCS"
/label="multiple cloning site from pUC9"
primer_bind complement(11063..11079)
/label="M13 fwd"
/label="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
