pAAV-RC Search name
pAAV-RC,Plasmid pAAV-RC,pAAV-RC vector
pAAV-RC Information
Promoter: Lac
Replicator: pUC ori, F1 ori
Plasmid classification: virus series, adenovirus related packaging carrier
Plasmid size: 7327bp
Prokaryotic resistance: Amp
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: M13F:TGTAAAACGACGGCCAGT
3'sequencing primers: M13R:CAGGAAACAGCTATGACC
pAAV-RC Description
pAAV-RC plasmid contains the AAV-2 rep and cap genes, encoding replication proteins and viral capsid structural proteins, respectively. Establishing the proper expression levels of rep and cap gene products is an important step in achieving high-titer virus production. pAAV-RC directs Rep and Cap expression from two different promoters, achieving optimal expression levels for each set of gene products.
pAAV-RC Sequence
LOCUS Exported 7327 bp ds-DNA circular SYN 27-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7327)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, August 27, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..7327
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 36..52
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 66..113
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FRT"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind complement(4548..4564)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(4705..5160)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5187..5291
/gene="bla"
/note="AmpR promoter"
CDS 5292..6152
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6323..6911
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7199..7220
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7235..7265
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 7273..7289
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7297..7313
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
1741 cgtgggcatg <
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